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Article is devoted to production and occupational exposure to «biological factor» for workers оf railway transport. Purpose - improving the system of occupational safety in railway transport under the influence of workers pathogenic organisms. To identify gaps in the system of assessment «biological factor» has been studied Russian normative documentation for a special assessment of working conditions and carried out long-term work on hygienic assessment of working conditions. The article defines the term «biological factor», justify the need for of risk assessment of the harmful factor, expand the list of sources and professional group of railway workers potentially exposed to «biological factor». In this article presents a device for protection and prophylaxis railway workers from biological factor. The principle of operation of the device is based on the integrated use of methods of ultrasonic treatment and ionization to disinfect the indoor air of railway stations, bus stations, subway stations. According to the results of studying the Russian regulatory documentation in the transportation of dangerous goods by railway, identified the main problems and their solutions, in terms of biological safety: formulated the term «biologically dangerous goods»; the necessity of creating a class or sub-class «biologically dangerous goods» and its implementation in the existing rules; developed a simple list of «biologically dangerous goods»; the possibility of the impact of these goods on railway workers.